Central Nervous System- Basic Neuropathology
Question No. 24. The Answer is: (A)- Buschino bodies.
Discussion:
Buschino body is an artifact due to prolonged submersion in formalin. Morphologically, they appear as irregular "cracks" that contains a small amount of pale bluish-gray amorphous material.
Lewy bodies are inclusion bodies that are found in Parkinson's disease and diffuse Lewy body disease. In Parkinson’s disease, Lewy bodies are found predominantly in substantia nigra. They are concentric, hyaline cytoplasmic inclusions with a clear halo around a dense central region on hematoxylin and eosin stain. Lewy bodies bear immunoreactivity for antibodies against neurofilament proteins, ubiqitin and alpha-synuclein.
Hirano bodies are eosinophilic, highly-refractile, rod-like structures (about 10 micrometers in thickness) adjacent to pyramidal neurons in the hippocampal formation in Alzheimer's disease, Pick's disease, and other neurodegenerative disorders. Hirano bodies often parallel the distribution of the granulovacuolar bodies of Simchowicz. Hirano bodies compose of microfilament-associated proteins including actin, alpha-actinin, and vinculin.
Lafora bodies are found in Lafora body disease and adult polyglucosan body disease, both are rare entities. Lafora bodies vary in size from 1 to 30 micron in diameter and one or more Lafora bodies may be present in the cytoplasm. They have a concentric target like lamination and are found in nerve cell processes and apparently free in the neuropil. Lafora bodies are PAS positive, diastases resistant, and Alcian blue positive. The core is more strongly stained than the rim. Lafora bodies are also basophilic, and variably metachromatic (with methyl violet or toluidine blue) inclusion bodies. As Lafora bodies are also found in liver, striated muscles, sweat glands, biopsy of these organs can facilitate diagnosis.
Marinesco bodies are eosinophilic ubiquitinated intranuclear inclusions found in pigmented neurons of the human substantia nigra and locus coeruleus. Traditionally they are not considered as pathological changes, more recent studies have indicated that marinesco bodies are associated with the age-associated degenerative changes in the substantia nigra and striatal loss of dopaminergic terminals 1. Woulfe J et al show colocalization, contiguity, and focally shared immunoreactivity between MBs and neuronal intranuclear rodlets (INRs). INRs are markedly decreased in the cortex of Alzheimer's disease, but not dementia with Lewy bodies 2.
Reference:
Beach TG, Walker DG, Sue LI, Newell A, Adler CC, Joyce JN. Substantia nigra Marinesco bodies are associated with decreased striatal expression of dopaminergic markers. J Neuropathol Exp Neurol. 2004;63:329-37.
Woulfe J, Gray D, Prichett-Pejic W, Munoz DG, Chretien M. Intranuclear rodlets in the substantia nigra: interactions with marinesco bodies, ubiquitin, and promyelocytic leukemia protein. J Neuropathol Exp Neurol. 2004;63:1200-7.