Histotechnology I- Miscellaneous
Question No. 13. The Answer is: (B) 1 and 3 are true.
1. The stain being used demonstrates the reticular fibers.
3. The staining mechanism involves reducing activated silver ion into silver metal.
Discussion: This photo demonstrated the reticulin fibers in the anterior pituitary. Reticular fibers can be demonstrated by Gordon and Sweet's method or Gomori's method. Both methods involve the reduction of silver ion into silver metal and its deposition on the reticulin fiber would turn them black. Verhoeff elastic stain involves hematoxylin with high concentration of ferric chloride and stains elastic fibers black.