Molecular Pathology- Miscellaneous I
Question No. 3. The Answer is: (A) Treatment by acids such as hydrochloric acid.
Discussion: Hydrogen bonds between complementary bases of the two strands of DNA are typically disrupted either by treatment with a strong alkali, or high temperature (~90oC). Dissociation of the two strands can be accomplished at lower temperatures with the use of an organic agent such as formamide.