Molecular Pathology- Miscellaneous I
Question No. 5. The Answer is: (D) Digest with specific restriction endonuclease(s).
Discussion: Comment: Extract DNA → Digest with restriction endonuclease (restriction enzyme) → Electrophorese in agarose gel → denature and depurinate DNA in gel → transfer to membrane → hybridize with probe → wash → detect signal. Additional steps after extraction of DNA and before cutting with restriction enzyme(s) may also include measuring the concentration and purity of the DNA (generally by spectrophotometry) and even running a check gel to see the integrity of the DNA.